Article Sidebar

Download
PDF
Statistic
Read Counter : 0 Download : 0

Downloads

Download data is not yet available.

Main Article Content

Abstract

Cancer remains one of the leading causes of global mortality, prompting the need for novel, effective, and affordable anticancer agents. Marine macroalgae, including Padina sp., are known to contain bioactive compounds with therapeutic potential. This study aimed to evaluate the anticancer potential of ethanolic extract of Padina sp. using the Brine Shrimp Lethality Test (BSLT) and compare it with taurine as a control compound. Phytochemical screening confirmed the presence of several secondary metabolites including alkaloids, flavonoids, phenolics, saponins, tannins, terpenoids, and steroids in the ethanolic extract. Toxicity assays using Artemia salina larvae revealed that both taurine and Padina sp. extract induced larval mortality in a dose-dependent manner. Regression analysis indicated LC50 values of 170.69 ppm for taurine and 168.52 ppm for the Padina sp. extract, categorizing both as having low toxicity levels. The observed larval mortality is likely associated with the bioactive compounds, particularly alkaloids, flavonoids, saponins, and tannins, which have been previously reported to exhibit cytotoxic and anticancer properties. These findings suggest that Padina sp. ethanolic extract has potential as a natural anticancer candidate and warrants further investigation using cancer cell lines and in vivo models.

Keywords

Padina sp. Brine Shrimp Lethality Test anticancer potential cytotoxicity taurine

Article Details

References

  1. Agata, A., Widiastuti, E. L., Susanto., Sutayrso, G. N., 2016. Respon histopatologis hepar mencit (Mus musculus) yang diinduksi benzo(α) piren terhadap pemberian taurin dan ekstrak daun sirsak (Annona muricata). Jurnal Natur Indonesia. 16(2): 54-63. DOI: 10.31258/jnat.16.2.54-63.
  2. American Cancer Society. 2019. Breast Cancer : Treating Breast Cancer, American Cancer Society, p. 1-120. DOI: 10.52214/vib.v9i.10309.
  3. Asraf, M.A. 2020. Phytochemicals as potential anticancer drugs: time to ponder nature’s bounty. BioMed Research International. Volume 2020, Article ID 8602879, 7 pages. DOI: 10.1155/2020/8602879.
  4. Aris, M. and Adriana, A. N. I., 2022. Uji LC50 ekstrak daun mentimun (Cucumis sativus L) teradap larva udang renik air asin (Artemia salina Leach) dengan menggunakan metode BSLT. Fito Medicine: Journal Pharmacy and Science. 14(1): 36-42. DOI: 10.47650/fito.v14i1.493.
  5. Calballo, J. L., Inda, Z. L. H., Perez, P., Gravalos, M. D. G., 2002. A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products. BMC Biotechnology. 2(1): 1-5.
  6. Fajarningsih, N.D., Januar, H.I, Nursid, M, Wikanta, T. 2006. Potensi antitumor ekstrak spons Crella papilata asal Taman Nasional Laut Kepulauan Seribu. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan: 1(1): 35-41.
  7. Haryani, T. S., Lohitasari, B., Triastinurmiatiningsih., 2019. Toxicity and compound identification of Padina australis extract. International Journal of Recent Technology and Engineering. 8(2): 79-82. DOI: 10.35940/ijrte.B1016.0782S719.
  8. Hoadley, K. A., Yau, C., Hinoue, T., Wolf, D. M., Lazar, A. J., Drill, E., Shen, R., Taylor, A. M., Cherniack, A. D., Thorsson, V., Akbani, R., Bowlby, R., Wong, C. K., Wiznerowicz, M., Sanchez-Vega, F., Robertson, A. G., Schneider, B. G., Lawrence, M. S., Noushmehr, H., Malta, T. M., Cancer Genome Atlas Network, Stuart, J. M., Benz, C. C., Laird, P. W., 2018. Cell- of-origin patterns dominate the molecular classification of 10.000 tumors from 33 types of cancer. PubMed. p. 291-304. DOI: 10.1016/j.cell.2018.03.022.
  9. Karim, H., Ahmad, A., Natzir, R., Massi, M. N., Arfah, R., Asmi, N., Karim, A., 2019. Isolation and identification of bioactive proteins from the brown algae Sargassum sp and their potential as anticancer agents. Journal of Physics: Conference Series. 1341(3): 1-9. DOI: 10.1088/1742-6596/1341/3/032009.
  10. Khadijah, K., Soekamto, N. H., Firdaus, F., Chalid, S. M. T., Syah, Y. M., 2021. Chemical composition, phytochemical constituent and toxicity of methanol extract of brown algae (Padina sp) from Puntondo Coast, Takalar (Indonesia). Journal of Food Quality and Hazard Control. 8(4): 178-185. DOI: 10.18502/jfqhc.8.4.8259.
  11. Kurniatanty, I., Tan, M. I., Ruml, T., Sumarsono, S. H., 2015. Potential cell proliferation inhibitor isolated from Indonesian brown algae (Phaeophyta). International Journal of Pharmacy and Pharmaceutical Sciences, 7(1): 140-143.
  12. Kurniawan, H. & Ropiqa, M., 2021. Uji toksisitas ekstrak etanol daun kelor kucing (Achalypha hispida Burm.f.) dengan metode brine shrimp lethality test (BSLT). Journal Syfa Sciences and Clinical Research. 3(1): 52-62. DOI: 10.37311/jsscr.v3i2.11398.
  13. Nuralifah., Parawansah., Nur, H., 2021. Uji Toksisitas akut ekstrak air dan ekstrak etanol daun kacapiring (Gardenia jasminoides Ellis) terhadat larva Artemia salina Leach dengan metode brine shrimp lethality test (BSLT). Indonesian Journal of Pharmaheutical Education. 1(2): 98-106. DOI: 10.37311/ijpe.v1i2.11462.
  14. Meyer, B.N., Ferrigni, N.R., Putnam, J.E., Jacobsen, L.B., Nichols, D.E., Mc Laughlin, J.L., 1982. Brine shrimp: a convenient general bioassay for active plant constituents, Planta Medica, 45(05), 31-34.
  15. Mondal,A, A. Gandhi, C. Fimognari, A.G. Atanasov, A.Bishayee. 2019. Alkaloids for cancer prevention and therapy: Current progress and future perspectives. European J. of Pharmacology. Vol, 858: 172472.
  16. Patel, R.P., Patel, M.P., Suthar, A.M. 2006. Spray drying technology. Indian Journal of Science and Technology 2(10): 44-47
  17. Roselyn, A.P., Widiastuti, E.L., Susanto, G.N., Sutyarso. 2016. Pengaruh pemberian taurin terhadap gambaran histopatologi paru mencit (Mus musculus) yang diinduksi karsinogen Benzo(α)Piren secara In Vivo. Jurnal Natur Indonesia 17(1): 22-32
  18. Rushdi, M. I., Abdel-Rahman, I. A. M., Saber, H., Attia, E. Z., Madkour, H. A., Abdelmohsen, U. R., 2021. A review on the pharmacological potential of genus Padina. South African Journal of Botany. 141, 37-48. DOI: 10.1016/j.sajb.2021.04.018.
  19. Subramaniam, S., Selvaduray, K. R., & Radhakrishnan , A. K,. 2019. Bioactive compounds: natural defense against cancer?. Biomolecules 2019. 9(758): 1-15. DOI: 10.3390/biom9120758.
  20. Tasmin, N., Erwin., Irawan, W. K., 2014. Isolasi, identifikasi dan uji toksisitas senyawa flavonoid fraksi kloroform dari daun terap (Artocarpus odoratissimus blanco). Jurnal Kimia Mulawarman. 12(1): 45-52.
  21. The Global Cancer Observatory. 2022. Indonesia. [online]. https://gco.iarc.fr/today/en/fact-sheets-populations#countries.
  22. Wagner, J.G., 1993. Pharmacokinetics for the pharmaceutical scientist. Lancarter-Basel: Technomic Pub.
  23. Wijayanti, N., Sudjarwo, G.W., Putra, O.N. 2020. Skrining fitokimia metabolit sekunder alga cokelat (Padina australis) dari Kepulauan Poteran Madura. Journal of Pharmaceutical Care AnwarMedika. 2 (2): 60-69.