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Abstract
Evidence identification methods are developing towards molecular DNA (Deoxyribonucleic acid) forensics for personal identification, tracing genetic relationships and tracking the source of biological material. The identification process using DNA has many variations, one of which is through Short Tandem Repeat (STR) analysis. This study aims to analyze DNA touch quality on face shields by giving using STR CODIS TH01. The sample consisted of 2 respondents determined by random sampling. Respondent I was used to measure DNA quality on day 1 and respondent II was used to measure DNA quality on day 7. The quality of DNA on face shields was different on day 1 and day 7. On day 1, the average DNA content was 1.29, while on day 7 the average DNA content was 1.22 with an average daily environmental temperature of 28.75oC and average daily humidity of 89.5%.The conclusion is DNA quality experienced changes in different exposure times as indicated by the average DNA levels on day 1 and day 7. Differences in DNA quality still provide good visualization results on gel electrophoresis so that it can still be used as identification material, especially forensic identification.
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References
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- Sessa, Francesco et al. 2019. “Touch DNA: Impact of Handling Time on Touch Deposit and Evaluation of Different Recovery Techniques: An Experimental Study.” Scientific Reports 9(1): 1–9.
- da Silva, Ricardo Henrique Alves, and Rogério Nogueira de Oliveira. 2008. “Forensic Anthropology and Molecular Biology: Independent or Complementary Sciences in Forensic Dentistry? An Overview.” Brazilian Journal of Oral Sciences 7(25): 1575–79.
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- Tobias, Samuel H.A., Guilherme S. Jacques, Ruth M. Morgan, and Georgina E. Meakin. 2017. “The Effect of Pressure on DNA Deposition by Touch.” Forensic Science International: Genetics Supplement Series 6: e12–14. http://dx.doi.org/10.1016/j.fsigss.2017.09.0 20.
- Tozzo, Pamela et al. 2022. “Touch DNA Sampling Methods: Efficacy Evaluation and Systematic Review.” International Journal of Molecular Sciences 23(24).
- Yudianto, Ahmad, and Soekry Erfan Kusuma. 2006. “DNA Isolation from Sweat Stain in Clothes as Forensic Identification Material.” Folia Medica Indonesia 42(4): 205.
- Yudianto, Ahmad, Simon Martin Manyanza Nzilibili, Pudji Harjanto, and Fery Setiawan. 2020. “The Use of Touch DNA Analysis in Forensic Identification Focusing on STR CODIS LOCI THO1, CSF1PO and TPOX.” Indian Journal of Forensic Medicine and Toxicology 14(3): 1692–96.
- Yudianto, Ahmad, and Yeti Eka Sispitasari. 2017. “Isolasi DNA Dari Bercak Urine Manusia Sebagai Bahan Alternatif Pemeriksaan Identifikasi Personal.” MPI (Media Pharmaceutica Indonesiana) 1(1): 53–61
References
Burrill, Julia et al. 2021. “Corneocyte Lysis and Fragmented DNA Considerations for the Cellular Component of Forensic Touch DNA.” Forensic Science International: Genetics 51(October 2020): 102428. https://doi.org/10.1016/j.fsigen.2020.10242 8.
Huang, Lien Hung et al. 2017. “The Effects of Storage Temperature and Duration of Blood Samples on DNA and RNA Qualities.” PLoS ONE 12(9): 1–13.
Kirkinen, Tuija et al. 2022. “Determination of Different Predictors Affecting DNA Concentration Isolated from Historical Hairs of the Finnhorse.” Journal of Archaeological Science: Reports 41(November 2021): 103262. https://doi.org/10.1016/j.jasrep.2021.10326 2.
Moore, Robert. 2013. “The Role of Computer Forensics in Criminal Investigations.” Crime Online (November): 81–94.
Nte, Ucheawaji Nathaniel, Ngboawaji Daniel Nte, Bribena Kelvin Enokie, and Onyeka Bienose. 2019. “) 2019 of Law.” Jils (Journal of Indonesian Legal Studies) 4(2). http://journal.unnes.ac.id/sju/index.php/jils.
Puspa, Egita Windrianatama et al. 2022. “Analysis of Time Exposure to DNA Touch Quality on Face Shield Using STR CODIS – TH01 and D18S51.” Jurnal Teknologi Laboratorium 10(2): 68–74.
Sessa, Francesco et al. 2019. “Touch DNA: Impact of Handling Time on Touch Deposit and Evaluation of Different Recovery Techniques: An Experimental Study.” Scientific Reports 9(1): 1–9.
da Silva, Ricardo Henrique Alves, and Rogério Nogueira de Oliveira. 2008. “Forensic Anthropology and Molecular Biology: Independent or Complementary Sciences in Forensic Dentistry? An Overview.” Brazilian Journal of Oral Sciences 7(25): 1575–79.
Sophian, Alfi, and Andi Syukur. 2021. “Analysis of Purity and Concentration of Isolated DNA in Making Raw DNA of Rat Species.” Eruditio : Indonesia Journal of Food and Drug Safety 1(2): 1–5.
Tasker, Esiri et al. 2020. “Efficacy of ‘Touch’ DNA Recovery and Room-Temperature Storage from Assault Rifle Magazines.” Legal Medicine 43(March 2019).
Tobias, Samuel H.A., Guilherme S. Jacques, Ruth M. Morgan, and Georgina E. Meakin. 2017. “The Effect of Pressure on DNA Deposition by Touch.” Forensic Science International: Genetics Supplement Series 6: e12–14. http://dx.doi.org/10.1016/j.fsigss.2017.09.0 20.
Tozzo, Pamela et al. 2022. “Touch DNA Sampling Methods: Efficacy Evaluation and Systematic Review.” International Journal of Molecular Sciences 23(24).
Yudianto, Ahmad, and Soekry Erfan Kusuma. 2006. “DNA Isolation from Sweat Stain in Clothes as Forensic Identification Material.” Folia Medica Indonesia 42(4): 205.
Yudianto, Ahmad, Simon Martin Manyanza Nzilibili, Pudji Harjanto, and Fery Setiawan. 2020. “The Use of Touch DNA Analysis in Forensic Identification Focusing on STR CODIS LOCI THO1, CSF1PO and TPOX.” Indian Journal of Forensic Medicine and Toxicology 14(3): 1692–96.
Yudianto, Ahmad, and Yeti Eka Sispitasari. 2017. “Isolasi DNA Dari Bercak Urine Manusia Sebagai Bahan Alternatif Pemeriksaan Identifikasi Personal.” MPI (Media Pharmaceutica Indonesiana) 1(1): 53–61