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Abstract

The mechanism of action of antioxidant is related to several diseases that cause health problems, such as: anti- inflammatory, anti-atherosclerotic, anti-thrombogenic and anti- tumor. This disease is caused by oxidative stress which occurs due to free radicals. Secondary metabolite compounds from medicines plants such as phenolic and flavonoids compounds are sources of antioxidant. The sungkai plant (P. canescens Jack) contains flavonoids, phenolic compounds, steroids, tannins, saponins and alkaloids. Therefore, this plant could be used as natural antioxidant source. The aim of the present study was to evaluated the antioxidant activity of the ethyl acetate extract of sungkai leaves (P. canescens Jack) using the DPPH method. The sungkai leaves powder was macerated with methanol for 3x24 hours and partitioned the concentrated extract with different solvents such as n-hexane, ethyl acetate, methanol and water. The ethyl acetate extract was selected to further fractionated using VLC method with n-hexane: ethyl acetate with gradient as eluent. The antioxidant activity was carried out using DPPH method with different concentration of 20, 40, 60, 80, and 100 ppm. The ability of antioxidant activity in the ethyl acetate extract of sungkai leaves was indicated by the change in DPPH colour from purple to yellow after incubation for 30 minutes. The ascorbic acid was used as positive control. The ethyl acetate extract was fractionated using VLC method to afford subfractions FEA, FEB, FEC, FED, and FEE. The antioxidant activity of FEA, FEB, FEC, FED, and FEE sub-fractions exhibited the strong antioxidant activity with the IC50 values of 0.848, 4.635, 3.277, 2.055, and 0.689 ppm, respectively. From the above findings, it is concluded that sungkai leaves have potential as natural antioxidant source particularly from ethyl acetate extract. However, the purification is still needed to identify the bioactive compounds which have potential as antioxidant activity.

Keywords

Antioxidant activity, Peronema Canescens, Sungkai Leaves.

Article Details

References

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