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Abstract

Binahong is a plant that contains active compounds and has potential as an anti-oxidant, anti- inflammatory, and anti-bacterial. The active compounds of binahong plants have been widely studied to play a role in the phases of the wound healing process. This study examined the phytochemicals, the potential of the extract as an antibacterial, and the effectiveness of binahong (Anredera cordifolia) gel on the diameter of full-thickness wounds in Rattus norvegicus rats. This type of research is in vitro and in vivo, using a true experimental post- test control design. Bacteria used in the study, Pseudomonas aeruginosa and Staphylococcus aureus, were tested using concentrations of binahong extract at 25%, 50%, 75%, and 100%. The research sample was Rattus norvegicus rats, which were divided into 5 treatment groups: negative control, positive control, and binahong gel doses of 10%, 20%, and 30%. The parameters used for the antibacterial test were the zone of inhibition and wound healing parameters seen from the measurement of wound diameter carried out on day 14. Statistical tests used descriptive analysis and one-way ANOVA test. The phytochemical screening test of binahong (Anredera cordifolia) extract contains alkaloid, flavonoid, triterpenoid, steroid, saponin, and tannin compounds. Binahong extract effectively inhibits Pseudomonas aeruginosa and Staphylococcus aureus bacteria. There is a clear zone, which is the zone of inhibition. The most effective concentration of binahong extract in inhibiting Pseudomonas aeruginosa bacteria was obtained at a concentration of 75% and Staphylococcus aureus at a concentration of 25%. The results of the one-way anova test showed that there was a significant difference in the mean wound diameter between treatment groups (p<0.05). Games Howell's post hoc test found that the dose of binahong gel that is effective in wound healing is found in the 10% dose of binahong gel. Binahong contains active compounds of alkaloids, flavonoids, triterpenoids, steroids, saponins, and tannins that have the potential to be antibacterial and proven in wound healing, as seen from the decrease in wound diameter.

Keywords

Phytochemical, Binahong, Antibacterial, Wound Diameter

Article Details

References

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